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Figure 8.19 1 H-NMR assay. Lysyl tRNA synthetase (LysU) assay system. Conversion of ATP into Ap4 A is observed by 1 H-NMR spectroscopy when reaction is run in a 5mm NMR tube. 1 H signals of adenine ring are shifted up eld with conversion of ATP ( H 8.55 [H-2] and 8.29 [H-8] p.p.m.) to Ap4 A ( H 8.40 [H-2] and 8.18 [H-8] p.p.m.). Rates of conversion are followed by changes in appropriate 1 H-NMR signal peak areas as a function of time (illustration adapted from Theoclitou et al., 1996, Fig. 2). asp.net ean 13 EAN - 13 ASP . NET Control - EAN - 13 barcode generator with free ...
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